6QRQ

Apo conformation of chemotaxis sensor ODP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-12
Detector	ADSC QUANTUM 210
Wavelength(s)	1.2800
Spacegroup name	P 21 21 21
Unit cell lengths	84.650, 110.725, 110.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.540 - 2.562
Rwork	0.191
R-free	0.25450
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.540	2.654
High resolution limit [Å]	2.562	2.562
Rmerge	0.162
Rmeas	0.124
Rpim	0.050
Number of reflections	33480
<I/σ(I)>	7.6
Completeness [%]	94.0
Redundancy	11.6
CC(1/2)	0.997	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M Hepes pH 7.5, 7% Isopropanol, 23% polyethyleneglycol 4000