6QRN

Galectin-10 complexed with ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	P 65 2 2
Unit cell lengths	49.139, 49.139, 261.025
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.500 - 1.400
R-factor	0.172
Rwork	0.171
R-free	0.19200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xrg
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	TRUNCATE
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.400	1.400
Rmeas	0.107	0.935
Number of reflections	37263	5725
<I/σ(I)>	25.24	3.38
Completeness [%]	97.2	95
Redundancy	18.9	18.4
CC(1/2)	0.999	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	PBS supplemented with 300 mM D-ribose. Cryoprotection: 25 % (w/v) PEG 400