6QQJ

Room temperature structure of the ground state of AtPhot2LOV2 recorded after an accumulated dose of 354 kGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	293
Detector technology	PIXEL
Collection date	2016-05-20
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.968
Spacegroup name	P 43 21 2
Unit cell lengths	40.886, 40.886, 132.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.210 - 2.080
R-factor	0.24925
Rwork	0.247
R-free	0.29153
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eep
RMSD bond length	0.002
RMSD bond angle	1.239
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.070	2.150
High resolution limit [Å]	2.080	2.080
Number of reflections	7399	680
<I/σ(I)>	10.72	1.73
Completeness [%]	93.6	87.4
Redundancy	8.57
CC(1/2)	0.999	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	12-17% PEG8000 0.2 M Calcium acetate 0.1 M MES