6QQI

Cryogenic temperature structure of blue light-irradiated AtPhot2LOV2 recorded after an accumulated dose of 24 kGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 43 21 2
Unit cell lengths	40.324, 40.324, 131.278
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.550 - 1.700
R-factor	0.19619
Rwork	0.194
R-free	0.23886
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eep
RMSD bond length	0.004
RMSD bond angle	1.594
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.550	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	12655	1202
<I/σ(I)>	10.18	1.74
Completeness [%]	99.7	99
Redundancy	6.24	6.47
CC(1/2)	0.997	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	12-17% PEG8000 0.2 M Calcium Acetate 0.1 M MES