6QQI
Cryogenic temperature structure of blue light-irradiated AtPhot2LOV2 recorded after an accumulated dose of 24 kGy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 40.324, 40.324, 131.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.550 - 1.700 |
| R-factor | 0.19619 |
| Rwork | 0.194 |
| R-free | 0.23886 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eep |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.594 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.550 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 12655 | 1202 |
| <I/σ(I)> | 10.18 | 1.74 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 6.24 | 6.47 |
| CC(1/2) | 0.997 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 12-17% PEG8000 0.2 M Calcium Acetate 0.1 M MES |
