6QQ8

Cryogenic temperature structure of the fluorescent protein Cerulean recorded after an accumulated dose of 290 kGy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	51.110, 62.720, 70.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.810 - 1.460
R-factor	0.16505
Rwork	0.163
R-free	0.19689
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wso
RMSD bond length	0.011
RMSD bond angle	1.546
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0218)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.820	1.500
High resolution limit [Å]	1.460	1.460
Number of reflections	39910	2939
<I/σ(I)>	24.07	1.56
Completeness [%]	99.9	99.9
Redundancy	7.1	7.3
CC(1/2)	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100mM HEPES pH 7.0, 12% PEG8000, 100mM MgCl2