6QQ8
Cryogenic temperature structure of the fluorescent protein Cerulean recorded after an accumulated dose of 290 kGy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.110, 62.720, 70.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.810 - 1.460 |
| R-factor | 0.16505 |
| Rwork | 0.163 |
| R-free | 0.19689 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.546 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.820 | 1.500 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Number of reflections | 39910 | 2939 |
| <I/σ(I)> | 24.07 | 1.56 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.1 | 7.3 |
| CC(1/2) | 0.999 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM HEPES pH 7.0, 12% PEG8000, 100mM MgCl2 |
