6QQ4
Odorant-binding protein dmelOBP28a from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.500, 83.200, 45.600 |
| Unit cell angles | 90.00, 99.00, 90.00 |
Refinement procedure
| Resolution | 45.039 - 1.998 |
| R-factor | 0.1924 |
| Rwork | 0.190 |
| R-free | 0.23610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1org |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.039 | 2.050 |
| High resolution limit [Å] | 1.998 | 2.000 |
| Rmerge | 0.065 | |
| Rmeas | 0.066 | 0.854 |
| Number of reflections | 16958 | 1282 |
| <I/σ(I)> | 13.68 | 1.99 |
| Completeness [%] | 98.1 | 98.7 |
| Redundancy | 3.29 | 3.46 |
| CC(1/2) | 0.998 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.5 M sodium malonate, 0.1 M HEPES, 0.5% Jeffamine |
