6QPO

Adenovirus species D serotype 49 Fiber-Knob KO1 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97628
Spacegroup name	P 1 2 1
Unit cell lengths	105.170, 55.990, 116.030
Unit cell angles	90.00, 112.47, 90.00

Refinement procedure

Resolution	57.060 - 2.450
R-factor	0.19577
Rwork	0.193
R-free	0.25845
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1knb
RMSD bond length	0.012
RMSD bond angle	2.002
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.070	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.097	1.274
Number of reflections	45958	3349
<I/σ(I)>	9.2	1.2
Completeness [%]	99.3	99.3
Redundancy	3.7	3.7
CC(1/2)	0.994	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	Molecular Dimensions, PACT Pemier Screen, condition D05 (0.1 M MMT [Malic acid, MES, Tris], pH 8.0, 25 % w/v PEG 1500)