6QPN
Adenovirus species D serotype 49 Fiber-Knob
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 106.830, 56.280, 115.700 |
| Unit cell angles | 90.00, 112.95, 90.00 |
Refinement procedure
| Resolution | 106.540 - 2.740 |
| R-factor | 0.2136 |
| Rwork | 0.211 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1knb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.688 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 106.540 | 106.540 | 2.810 |
| High resolution limit [Å] | 2.740 | 12.250 | 2.740 |
| Rmerge | 0.151 | 0.082 | 1.633 |
| Rmeas | 0.176 | 0.097 | 1.910 |
| Rpim | 0.090 | 0.051 | 0.979 |
| Total number of observations | 122219 | 1299 | 9179 |
| Number of reflections | 33350 | 409 | 2461 |
| <I/σ(I)> | 6.8 | 18.1 | 1 |
| Completeness [%] | 99.1 | 96.3 | 98.9 |
| Redundancy | 3.7 | 3.2 | 3.7 |
| CC(1/2) | 0.988 | 0.981 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | PACT Premier condition D5 (0.1M MMT [Malic acid, MES, Tris], pH8.0, 25% w/v PEG 1500) |
