6QPM

Adenovirus serotype 10 Fiber-Knob

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91587
Spacegroup name	P 63
Unit cell lengths	183.570, 183.570, 94.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	79.490 - 3.390
R-factor	0.20096
Rwork	0.199
R-free	0.23242
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uxe
RMSD bond length	0.008
RMSD bond angle	1.695
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.490	3.580
High resolution limit [Å]	3.390	3.390
Rmerge	0.100	2.455
Rmeas	0.104	2.569
Number of reflections	25265
<I/σ(I)>	15.5
Completeness [%]	99.6
Redundancy	11.3
CC(1/2)	0.999	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M Sodium nitrate 0.1 M Bis-Tris propane 7.5 20 % w/v PEG 3350