6QPJ
Human CLOCK PAS-A domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-08-21 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 45.990, 45.360, 75.675 |
| Unit cell angles | 90.00, 93.46, 90.00 |
Refinement procedure
| Resolution | 25.063 - 2.315 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.24250 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.001 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 6784 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20 mM MES pH 6.5 0.1M Magnesium sulfate 20 % PEG 3350 |
