6QP5
Apo Human Calcium/Calmodulin-dependent kinase type 1D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 66.110, 45.790, 107.931 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 34.620 - 1.900 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jc6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.793 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1682) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.620 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.451 |
| Rmeas | 0.088 | 0.532 |
| Rpim | 0.041 | 0.276 |
| Total number of observations | 107590 | |
| Number of reflections | 24548 | 1444 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.1 | 93.3 |
| Redundancy | 4.4 | 3.6 |
| CC(1/2) | 0.996 | 0.487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG4000 -- 0.2M lithium sulfate -- 0.1M tris pH 8.5 |
