6QNV

Fibrinogen-like globe domain of Human Tenascin-C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	77.829, 77.829, 71.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.040 - 1.400
Rwork	0.164
R-free	0.18200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fid
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.930	72.060	1.220
High resolution limit [Å]	1.200	3.260	1.200
Rmeas	0.078	0.038	2.807
Rpim	0.016	0.008	0.769
Number of reflections	69462	3786	3391
<I/σ(I)>	18.5	71.2	1
Completeness [%]	100.0	100	99.2
Redundancy	23.4	23.7	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Morpheus 0.09M NPS, 0.1M Buffer System 3 pH = 8.5, 50% v/v Precipitant Mix 1