6QNP

CLATHRIN HEAVY CHAIN N-TERMINAL DOMAIN BOUND TO GTSE1 LIDL MOTIF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97793
Spacegroup name	P 1 21 1
Unit cell lengths	95.090, 90.150, 100.230
Unit cell angles	90.00, 110.12, 90.00

Refinement procedure

Resolution	49.190 - 2.700
R-factor	0.2434
Rwork	0.241
R-free	0.29140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m5t
Data reduction software	XFIT
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.190	49.190	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.141	0.026	1.890
Rmeas	0.153	0.028	2.052
Total number of observations	299788
Number of reflections	43602	520	3201
<I/σ(I)>	10.39	35.92	1.05
Completeness [%]	99.2	98.5	99
Redundancy	6.876	7.019	6.609
CC(1/2)	0.998	1.000	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	12% PEG 4000, 0.1M SODIUM ACETATE, 0.1M HEPES pH 7.0