6QNN

CLATHRIN HEAVY CHAIN N-TERMINAL DOMAIN BOUND TO GTSE1 LIDL MOTIF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99993
Spacegroup name	P 43
Unit cell lengths	51.987, 51.987, 154.094
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.259 - 2.030
R-factor	0.2418
Rwork	0.240
R-free	0.28390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1utc
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.259	49.259	2.080
High resolution limit [Å]	2.030	9.080	2.030
Rmerge	0.065	0.028	2.034
Rmeas	0.068	0.029	2.114
Number of reflections	26009	301	1929
<I/σ(I)>	18.24	62.58	1.73
Completeness [%]	98.8	99.3	98.2
Redundancy	13.639	14.223	13.432
CC(1/2)	1.000	0.999	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	20% PEG 6000, 0.1M TRIS pH 8.0