6QNC
Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 0.1 s timepoint
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2018-10-30 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 0.9778 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 94.630, 99.110, 87.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.977 - 1.897 |
R-factor | 0.1641 |
Rwork | 0.163 |
R-free | 0.19020 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.973 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.977 | 1.970 |
High resolution limit [Å] | 1.897 | 1.900 |
Number of reflections | 32696 | |
<I/σ(I)> | 6.3 | |
Completeness [%] | 99.5 | |
Redundancy | 168.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | 35% (w/v) PEG3350, 200 mM lithium sulfate and 10 mM Hepes/NaOH pH 7.5 |