6QNA
Structure of bovine anti-RSV hybrid Fab B13HC-B4LC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.440, 76.270, 130.030 |
| Unit cell angles | 89.66, 87.35, 88.60 |
Refinement procedure
| Resolution | 65.617 - 2.620 |
| R-factor | 0.2214 |
| Rwork | 0.220 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qn9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.547 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3386: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.900 | 2.670 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.050 | 0.931 |
| Number of reflections | 49218 | 2446 |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 98.2 | 98.1 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.999 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 28.0% w/v Polyethylene Glycol Monomethyl Ether 2000 0.1 M bis-Tris |
