6QN9

Structure of bovine anti-RSV Fab B4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 2
Unit cell lengths	78.335, 134.873, 42.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.700 - 1.890
R-factor	0.2294
Rwork	0.228
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dgg
RMSD bond length	0.003
RMSD bond angle	0.662
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((dev_3386: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.700	1.920
High resolution limit [Å]	1.890	1.890
Rmerge	0.129
Number of reflections	36744	1790
<I/σ(I)>	11.5	2.2
Completeness [%]	100.0	98.6
Redundancy	23.6
CC(1/2)	1.000	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M Ammonium Sulphate 25.0% w/v Polyethylene Glycol 3350 0.1 M HEPES