6QMS
NF-YB/C Heterodimer in Complex with NF-YA-derived Peptide Stabilized with C11-Hydrocarbon Linker
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.977970 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.950, 49.760, 74.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.060 - 1.800 |
| R-factor | 0.2089 |
| Rwork | 0.208 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4csr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.251 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.060 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 17408 | 2544 |
| <I/σ(I)> | 20.16 | 2.15 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 12.4 | 12.8 |
| CC(1/2) | 0.999 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M Tris pH8.5 0.2 M MgCl2 18% PEG8000 |
