6QMP
NF-YB/C Heterodimer in Complex with NF-YA Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.900, 52.900, 134.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.920 - 2.000 |
| R-factor | 0.2005 |
| Rwork | 0.198 |
| R-free | 0.24060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4csr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 15380 | 2046 |
| <I/σ(I)> | 21.85 | 2.81 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 18.9 | 18.9 |
| CC(1/2) | 0.999 | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 0.1 M Tris pH9.0 0.2 M NaAc 30% PEG4000 |
