6QLY

IDOL FERM domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-21
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	I 41 2 2
Unit cell lengths	159.490, 159.490, 76.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.217
Rwork	0.216
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qij
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.390	56.390	2.460
High resolution limit [Å]	2.400	10.720	2.400
Rmerge	0.077	0.021	6.805
Rmeas	0.083	0.023	7.378
Rpim	0.030	0.009	2.793
Number of reflections	19675	266	1400
<I/σ(I)>	12.7
Completeness [%]	99.8	99.3	97.7
Redundancy	7.1	6.1	6.7
CC(1/2)	1.000	1.000	0.046

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100mM Tris pH 8.5, 20mM MgSO4, 4% (w/v) ethylene glycol, 12% (w/v) 2-Methyl-2,4-pentanediol (MPD)