6QLL
Crystal structure of F181H UbiX in complex with FMN and dimethylallyl monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 142.033, 142.033, 142.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.670 - 1.560 |
| R-factor | 0.1627 |
| Rwork | 0.162 |
| R-free | 0.18270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zaf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.169 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.670 | 1.620 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.086 | |
| Number of reflections | 33618 | |
| <I/σ(I)> | 8.52 | |
| Completeness [%] | 98.5 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | LMB screen (Molecular Dimensions) F2 16 % w/v PEG 4000, 20 % v/v glycerol, 0.1 M sodium citrate pH 5.8, 0.1 M ammonium sulphate |
