6QKS
Crystal Structure of the Fluoroacetate Dehalogenase RPA1163 - Tyr219Phe - Apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-05-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.880, 78.090, 85.060 |
| Unit cell angles | 90.00, 103.37, 90.00 |
Refinement procedure
| Resolution | 18.799 - 1.600 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.24240 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.800 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 68397 | |
| <I/σ(I)> | 21.19 | |
| Completeness [%] | 97.3 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18-20% PEG3350, 200 mM CaCl2, and 100 mM Tris-HCl pH 8.5 |
