6QKG
2-Naphthoyl-CoA Reductase(NCR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.450, 86.840, 96.870 |
| Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
| Resolution | 29.940 - 2.200 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ps9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 69139 | 6879 |
| <I/σ(I)> | 13.2 | 1.8 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 4 | 4.1 |
| CC(1/2) | 0.998 | 0.713 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277.15 | PEG 4000/3350, NaF, Silver bullet solution A12/BioD4 |
