6QK4

Lytic transglycosylase, LtgG, of Burkholderia pseudomallei.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9763
Spacegroup name	P 32 2 1
Unit cell lengths	59.570, 59.570, 168.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.093 - 1.730
R-factor	0.1777
Rwork	0.176
R-free	0.20880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g5d 2gae
RMSD bond length	0.017
RMSD bond angle	1.364
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.100	1.780
High resolution limit [Å]	1.730	1.730
Number of reflections	37097
<I/σ(I)>	16.1	2.6
Completeness [%]	100.0	100
Redundancy	16.3	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1 M Bis-tris propane (pH 7.5), containing 0.2 M potassium sodium tartrate and 14% PEG 8K