6QJ1

Crystal structure of the C. thermophilum condensin Smc2 ATPase head (crystal from I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-29
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	47.320, 107.980, 174.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.893 - 2.560
R-factor	0.2341
Rwork	0.233
R-free	0.26210
RMSD bond length	0.003
RMSD bond angle	0.629
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.893	45.893	2.570
High resolution limit [Å]	2.500	11.190	2.500
Rmerge	0.082	0.042	1.854
Rmeas	0.084	0.043	1.916
Number of reflections	31694	420	2240
<I/σ(I)>	20.09	48.74	1.5
Completeness [%]	99.8	98.6	97.6
Redundancy	16.095	11.229	15.642
CC(1/2)	0.999	0.999	0.515

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18% (w/v) PEG3350 0.2 M Succinate pH 7.0 2 mM MnCl2