6QJ0

Crystal structure of the C. thermophilum condensin Smc2 ATPase head (crystal form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 65
Unit cell lengths	93.745, 93.745, 117.971
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	66.879 - 2.000
R-factor	0.1907
Rwork	0.189
R-free	0.22150
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	81.186	81.186	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge		0.064	1.649
Rmeas	0.103	0.067	1.733
Rpim	0.032	0.021	0.533
Total number of observations	409294
Number of reflections	39710	1299	5768
<I/σ(I)>	12.3	6.8	0.5
Completeness [%]	100.0	99.9	100
Redundancy	10.3	10.1	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10-17 % (w/v) PEG 8,000, 0.1 M Na cacodylate pH 6.0