6QIM
Structure of AtPIP2;4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 218.985, 218.985, 100.637 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.636 - 3.700 |
| R-factor | 0.3307 |
| Rwork | 0.328 |
| R-free | 0.35610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jc6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.809 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER (Phenix) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.636 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Number of reflections | 15438 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 98.2 | |
| Redundancy | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 277 | 1.5 M sodium formate, 0.05 M sodium cacodylate pH 5.5, 30% (v/v) PEG400, 3% w/v 6-Aminohexanoic acid |
