6QHJ

High-resolution crystal structure of calcium- and sodium-bound mouse Olfactomedin-1 beta-propeller domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-18
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	I 2 2 2
Unit cell lengths	61.794, 79.631, 111.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.733 - 1.250
R-factor	0.125
Rwork	0.124
R-free	0.14130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5amo
RMSD bond length	0.010
RMSD bond angle	1.168
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.760	1.290
High resolution limit [Å]	1.250	1.250
Rmerge	0.084	0.908
Rmeas	0.092	1.188
Rpim	0.037	0.751
Number of reflections	72028	4675
<I/σ(I)>	13.7	1
Completeness [%]	94.6	63.9
Redundancy	5.8	2.4
CC(1/2)	0.998	0.445

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 M Tris pH 8.5 8 % (w/v) PEG 8000