6QHG

Structure of the cap-binding domain of Rift Valley Fever virus L protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	31.580, 53.681, 135.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.133 - 1.483
R-factor	0.1539
Rwork	0.152
R-free	0.19350
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.904
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.990	1.536
High resolution limit [Å]	1.483	1.483
Rmerge	0.113	0.403
Rmeas	0.123	0.437
Rpim	0.048	0.168
Number of reflections	39006
<I/σ(I)>	10
Completeness [%]	99.0
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24% PEG 2000 MME, 200 mM Trimethylamine N-oxide, 2 mM TCEP, 5 mM dithiothreitol, 2 mM m7GTP and 100 mM Tris, pH 8.5