6QH7

AP2 clathrin adaptor mu2T156-phosphorylated core with two cargo peptides in open+ conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	P 41 21 2
Unit cell lengths	182.177, 182.177, 211.602
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	110.000 - 3.400
R-factor	0.20986
Rwork	0.208
R-free	0.24815
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.493
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.000	3.500
High resolution limit [Å]	3.400	3.400
Rmerge	0.280
Rmeas	0.290
Number of reflections	49558
<I/σ(I)>	8.8
Completeness [%]	99.9	99.2
Redundancy	13.88
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	7.5% PVA, 9% 1-propanol, 90 mM Hepes pH 7.4, 100 mM guanidine hydrochloride