6QFR

Structure of the mitogen activated kinase kinase 7 dfg-out conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	61.300, 68.800, 84.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.768 - 2.300
R-factor	0.2264
Rwork	0.224
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.339
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.768	2.400
High resolution limit [Å]	2.300	2.300
Rmeas	0.060	0.786
Number of reflections	16475	1914
<I/σ(I)>	29.65	5.01
Completeness [%]	99.8	99.1
Redundancy	12.2	11.2
CC(1/2)	0.999	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M Sodiumcitrate 20% PEG3350