6QFO
EngBF DARPin Fusion 9b 3G124
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 65 |
| Unit cell lengths | 193.470, 193.470, 123.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.510 - 2.300 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.20400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zxq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.470 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.191 | 4.085 |
| Number of reflections | 116670 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 21.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.9 | 277.15 | MPD, PEG 20,000, MES, sodium chloride, manganese chloride |
