6QFK
EngBF DARPin Fusion 4b G10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 192.893, 192.893, 122.922 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.250 - 2.000 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zxq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.330 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.254 | 2.903 |
| Number of reflections | 174930 | |
| <I/σ(I)> | 13.46 | |
| Completeness [%] | 100.0 | |
| Redundancy | 20.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.6 | 277.15 | MPD, PEG 20,000, MES, sodium chloride, manganese chloride |
