6QFJ
MamB CTD magnetosome protein [Desulfamplus magnetovallimortis BW-1]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.932 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.973, 47.070, 74.626 |
| Unit cell angles | 74.19, 84.86, 84.21 |
Refinement procedure
| Resolution | 23.910 - 2.130 |
| R-factor | 0.20145 |
| Rwork | 0.200 |
| R-free | 0.23453 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MamM |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.838 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.910 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 3384 | |
| <I/σ(I)> | 23.7 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.6 | 293 | 20% PEG 3,350 0.1M Tris pH 8.6 0.2M Ammonium acetate |
