6QER
Apo Form Of ComR From S. Thermophilus in space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980105 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 271.790, 73.720, 78.310 |
| Unit cell angles | 90.00, 104.97, 90.00 |
Refinement procedure
| Resolution | 44.240 - 2.465 |
| R-factor | 0.2324 |
| Rwork | 0.231 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5juf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.240 | 2.553 |
| High resolution limit [Å] | 2.465 | 2.465 |
| Rmeas | 0.109 | |
| Number of reflections | 105306 | 5173 |
| <I/σ(I)> | 14.34 | 2.35 |
| Completeness [%] | 99.6 | 96.05 |
| Redundancy | 6.89 | 6.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.05M Magnesium acetate 10% PEG8000 0.1M Sodium acetate |
