6QEK
Putative membrane tansporter, magnetosome protein MamM CTD [Desulfamplus magnetovallimortis BW-1]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 61 |
| Unit cell lengths | 47.019, 47.019, 130.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.720 - 1.950 |
| R-factor | 0.20958 |
| Rwork | 0.207 |
| R-free | 0.27160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MamM CTD MSR-1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.061 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.720 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.057 | |
| Number of reflections | 13784 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 25% PEG 3,350 0.1M BIS-TRIS pH 5.5 0.2M Sodium chloride |
