6QDK
Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 86.910, 86.910, 718.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.962 - 3.400 |
| R-factor | 0.2887 |
| Rwork | 0.287 |
| R-free | 0.31890 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.490 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmeas | 0.176 | 0.848 |
| Number of reflections | 41611 | |
| <I/σ(I)> | 12 | 1.9 |
| Completeness [%] | 99.6 | 96.2 |
| Redundancy | 18.2 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 77 mM HEPES pH 8.0 3.8 % Tacsimate 7.7% PEG MME 600 231 mM (NH4)2SO4 |
