6QCG

PCNA complex with Cdt1 N-terminal PIP-box peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0003
Spacegroup name	P 21 21 21
Unit cell lengths	76.585, 143.173, 173.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.210 - 3.400
R-factor	0.20826
Rwork	0.206
R-free	0.25308
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.702
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	3.630
High resolution limit [Å]	3.400	3.400
Number of reflections	26813
<I/σ(I)>	7.6	1.9
Completeness [%]	99.3	99.2
Redundancy	3.4
CC(1/2)		0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	300	15% (w/v) PEG 400, 100 mM calcium acetate, 100 mM MES/HCl