6QBY
Crystal structure of VASH 2 in complex with SVBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-06 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 119.887, 119.887, 102.636 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 103.830 - 2.090 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.830 | 2.345 |
| High resolution limit [Å] | 2.090 | 2.113 |
| Number of reflections | 30821 | 1542 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 99.9 | 78.5 |
| Redundancy | 10.9 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 0.2M Sodium formate 0.1M Bis-Tris propane pH 6.5 20% PEG3350 |
