6QBQ

structure of the core domaine of Knr4, an intrinsically disordered protein from Saccharomyces cerevisiae - mutant S200A S203A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 62
Unit cell lengths	102.816, 102.816, 92.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.000 - 2.350
R-factor	0.1963
Rwork	0.195
R-free	0.22398
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j1b
RMSD bond length	0.007
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.490
High resolution limit [Å]	2.350	2.350
Rmeas	0.056	1.127
Number of reflections	23243	3685
<I/σ(I)>	19.2	1.8
Completeness [%]	99.8	99.9
Redundancy	7.1	6.9
CC(1/2)	0.999	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	PEG 3000 - 6000 15-24 % (w/v) Bicine buffer 0.1 M