6QBP

structure of the core domaine of Knr4, an intrinsically disordered protein from Saccharomyces cerevisiae - mutant S203D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976256
Spacegroup name	P 62
Unit cell lengths	102.949, 102.949, 93.139
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.090 - 2.400
R-factor	0.19216
Rwork	0.191
R-free	0.21863
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j1b
RMSD bond length	0.006
RMSD bond angle	1.028
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.170	2.540
High resolution limit [Å]	2.400	2.400
Rmeas	0.043	1.115
Number of reflections	21807	3415
<I/σ(I)>	17.6	1
Completeness [%]	99.1	97.2
Redundancy	3.5	2.5
CC(1/2)	0.999	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	PEG 3000 - 6000 15-24 % (w/v) Bicine buffer 0.1 M