6QBO

structure of the core domaine of Knr4, an intrinsically disordered protein from Saccharomyces cerevisiae - mutant S203A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.072274
Spacegroup name	P 62
Unit cell lengths	103.104, 103.104, 93.565
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.190 - 2.750
R-factor	0.1867
Rwork	0.185
R-free	0.21355
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j1b
RMSD bond length	0.007
RMSD bond angle	1.105
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.200	2.800
High resolution limit [Å]	2.750	2.750
Rmeas	0.074	1.488
Number of reflections	14760	765
<I/σ(I)>	14.4	1.1
Completeness [%]	99.8	98.2
Redundancy	5.7	5
CC(1/2)	0.999	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	PEG 3000 - 6000 15-24 % (w/v) Bicine buffer 0.1 M