6QAP
Structure of the human aldehyde dehydrogenase 9A1 in C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.290, 160.030, 84.600 |
| Unit cell angles | 90.00, 91.09, 90.00 |
Refinement procedure
| Resolution | 47.740 - 2.300 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qak |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.740 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.152 | |
| Number of reflections | 96851 | |
| <I/σ(I)> | 9.89 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 14% PEG 4K, 0.1 M sodium citrate pH 5.6 |
