6QA7

Glycogen Phosphorylase b in complex with 29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION NOVA
Temperature [K]	293
Detector technology	CCD
Collection date	2018-05-28
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.5419
Spacegroup name	P 43 21 2
Unit cell lengths	128.212, 128.212, 115.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.700 - 2.360
R-factor	0.14378
Rwork	0.141
R-free	0.19099
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.008
RMSD bond angle	1.212
Data reduction software	CrysalisPro
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.700	2.450
High resolution limit [Å]	2.360	2.360
Number of reflections	35956	3168
<I/σ(I)>	15.1	2.3
Completeness [%]	90.2	76.8
Redundancy	5.7	4.1
CC(1/2)	0.996	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10 mM BES buffer