6QA6

Glycogen Phosphorylase b in complex with 30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION NOVA
Temperature [K]	293
Detector technology	CCD
Collection date	2018-05-28
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.5419
Spacegroup name	P 43 21 2
Unit cell lengths	128.161, 128.161, 115.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.690 - 2.400
R-factor	0.14366
Rwork	0.141
R-free	0.19894
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.527
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.690	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	33997	2975
<I/σ(I)>	16.5	3.3
Completeness [%]	90.3	76.8
Redundancy	5.8	4.2
CC(1/2)	0.997	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10 mM BES buffer