6QA1
ERK2 mini-fragment binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-02 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.654, 69.969, 60.040 |
| Unit cell angles | 90.00, 108.54, 90.00 |
Refinement procedure
| Resolution | 26.500 - 1.580 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.20300 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.650 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmeas | 0.048 | 0.203 |
| Number of reflections | 38645 | 159 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 73.6 | 4.1 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 0.2M (NH4)2SO4 33% MPEG 2000 0.02M Mercaptoethanol 0.1M pH=7.2 HEPES/NaOH |
