6Q9M
Central Fibronectin-III array of RIM-binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.095, 80.547, 93.077 |
| Unit cell angles | 90.00, 99.03, 90.00 |
Refinement procedure
| Resolution | 41.569 - 2.453 |
| R-factor | 0.2133 |
| Rwork | 0.211 |
| R-free | 0.25960 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.089 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmeas | 0.050 | 0.710 |
| Number of reflections | 27284 | 4373 |
| <I/σ(I)> | 18.2 | 2.14 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.998 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.4M KH2PO4, 18% PEG 3350 |
