6Q8O
Respiratory complex I from Thermus thermophilus with bound Piericidin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.140, 336.590, 262.790 |
| Unit cell angles | 90.00, 100.41, 90.00 |
Refinement procedure
| Resolution | 49.164 - 3.605 |
| R-factor | 0.2194 |
| Rwork | 0.218 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hea |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.804 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13rc1_2961: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.164 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.153 | 1.239 |
| Rpim | 0.133 | 1.106 |
| Number of reflections | 182595 | 26923 |
| <I/σ(I)> | 4.5 | 0.7 |
| Completeness [%] | 96.1 | 97.2 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 296 | 100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside |
