6Q8M
GH10 endo-xylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97965 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.342, 74.073, 213.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.090 - 1.420 |
| R-factor | 0.1489 |
| Rwork | 0.148 |
| R-free | 0.17049 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u7b |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.934 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.090 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.071 | |
| Rpim | 0.032 | 1.090 |
| Number of reflections | 142509 | |
| <I/σ(I)> | 15.7 | 2.3 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium/potassium phosphate (pH 6.9) |
