6Q88
RT structure of HEWL at 5 kGy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2016-09-16 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9219 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.300, 79.300, 38.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.825 - 1.740 |
| R-factor | 0.176936877007 |
| Rwork | 0.175 |
| R-free | 0.19944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ydg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.765 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 13310 | 802 |
| <I/σ(I)> | 7.14 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 195.8 | 106.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4 | 293 | 20 % NaCl 6 % PEG 6000 150 mM ammonium acetate pH 4.0 |
